What you can do in the space:
- Wet everything to force germination because it has a high resilience when it is dried (you even have spores that are resistant to radiation). Do that for 4 hours (approximately, depending on type of contamination, look at the time it germinates - more info to be found on that).
- Then you clean everything with bleach bombing (3-5%, the strongest the contamination the highest the grade) OR H2O2 - oxygen-water (used carefully and never mix both).
Make a checklist from the substrate to the environment to understand where the contamination comes from.
- Check the substrate and the material
- Check the bag or the container
- Measure the pH
- Check for moister
- Check control group: set up control groups
Method to minimize contamination:
Bring the mycelium from the bag to pots. This will allow you to only work with one pot at the time and avoid to open and close the mycelium bag each time. The best is to only work with the quantity of mycelium needed.
- Sterilize the pots in the autoclave with aluminium on the top
- Wait for the pots to cool down
- Put on the Bunsen brander
- Disinfect your hands and arms with alcohol
- Disinfect the table with alcohol
- Disinfect the outside of the pots with alcohol and place 1 on the table
- Disinfect the spoon with alcohol and place in over the Bunsen brander
- Disinfect the outside of the mycelium bag with alcohol
- Place the mycelium bag on the table with the opening to the Bunsen brander
- Open the mycelium bag with a disinfected scissor
- Disinfect the inside opening of the mycelium bag with alcohol carefully
- Cut out pieces of the mycelium and put in the pots
- Disinfect the ring of the pot, the spoon and the inside of the bag every time
- Close the pot every time.